全文获取类型
收费全文 | 1512篇 |
免费 | 157篇 |
出版年
2021年 | 28篇 |
2019年 | 17篇 |
2018年 | 12篇 |
2015年 | 45篇 |
2014年 | 49篇 |
2013年 | 55篇 |
2012年 | 72篇 |
2011年 | 62篇 |
2010年 | 47篇 |
2009年 | 36篇 |
2008年 | 53篇 |
2007年 | 56篇 |
2006年 | 66篇 |
2005年 | 57篇 |
2004年 | 49篇 |
2003年 | 36篇 |
2002年 | 37篇 |
2001年 | 44篇 |
2000年 | 39篇 |
1999年 | 55篇 |
1998年 | 24篇 |
1996年 | 17篇 |
1993年 | 14篇 |
1992年 | 33篇 |
1991年 | 15篇 |
1990年 | 29篇 |
1989年 | 26篇 |
1988年 | 22篇 |
1987年 | 16篇 |
1986年 | 28篇 |
1985年 | 26篇 |
1984年 | 20篇 |
1983年 | 19篇 |
1982年 | 18篇 |
1981年 | 20篇 |
1980年 | 17篇 |
1979年 | 30篇 |
1977年 | 19篇 |
1976年 | 12篇 |
1975年 | 26篇 |
1974年 | 25篇 |
1973年 | 31篇 |
1972年 | 21篇 |
1971年 | 23篇 |
1970年 | 20篇 |
1969年 | 19篇 |
1968年 | 12篇 |
1967年 | 17篇 |
1966年 | 15篇 |
1965年 | 13篇 |
排序方式: 共有1669条查询结果,搜索用时 15 毫秒
991.
Identification of novel biomass‐degrading enzymes from genomic dark matter: Populating genomic sequence space with functional annotation 下载免费PDF全文
992.
993.
994.
995.
Kristin M. Perrone-McGovern Patrícia Oliveira-Silva Stephanie Simon-Dack Erin Lefdahl-Davis David Adams John McConnell Desiree Howell Ryan Hess Andrew Davis Óscar F. Gonçalves 《Applied psychophysiology and biofeedback》2014,39(1):19-25
The present research builds upon the extant literature as it assesses psychophysiological factors in relation to empathy, conflict resolution, and romantic relationship satisfaction. In this study, we examined physiological reactivity of individuals in the context of emotionally laden interactions with their romantic partners. Participants (N = 31) completed self-report measures and attended in-person data collection sessions with their romantic partners. Participants were guided through discussions of problems and strengths of their relationships in vivo with their partners while we measured participants’ skin conductance level (SCL) and interbeat interval (IBI) of the heart. We hypothesized that participants’ level of empathy towards their partners would be reflected by physiological arousal (as measured by SCL and IBI) and relationship satisfaction, such that higher levels of empathy would be linked to changes in physiological arousal and higher relationship satisfaction. Further, we hypothesized that differences would be found in physiological arousal (as measured by SCL and IBI) based on the type of conflict resolution strategy used by participants. Finally, we hypothesized that differences would be found in empathy towards partner and relationship satisfaction based on the type of conflict resolution strategies used by participants. Results partially supported hypotheses and were discussed in light of existing knowledge based on empirical and theoretical sources. 相似文献
996.
997.
Daniel G. Tang Keyur B. Shah Micheal L. Hess Vigneshwar Kasirajan 《Journal of visualized experiments : JoVE》2014,(89)
With advances in technology, the use of mechanical circulatory support devices for end stage heart failure has rapidly increased. The vast majority of such patients are generally well served by left ventricular assist devices (LVADs). However, a subset of patients with late stage biventricular failure or other significant anatomic lesions are not adequately treated by isolated left ventricular mechanical support. Examples of concomitant cardiac pathology that may be better treated by resection and TAH replacement includes: post infarction ventricular septal defect, aortic root aneurysm / dissection, cardiac allograft failure, massive ventricular thrombus, refractory malignant arrhythmias (independent of filling pressures), hypertrophic / restrictive cardiomyopathy, and complex congenital heart disease. Patients often present with cardiogenic shock and multi system organ dysfunction. Excision of both ventricles and orthotopic replacement with a total artificial heart (TAH) is an effective, albeit extreme, therapy for rapid restoration of blood flow and resuscitation. Perioperative management is focused on end organ resuscitation and physical rehabilitation. In addition to the usual concerns of infection, bleeding, and thromboembolism common to all mechanically supported patients, TAH patients face unique risks with regard to renal failure and anemia. Supplementation of the abrupt decrease in brain natriuretic peptide following ventriculectomy appears to have protective renal effects. Anemia following TAH implantation can be profound and persistent. Nonetheless, the anemia is generally well tolerated and transfusion are limited to avoid HLA sensitization. Until recently, TAH patients were confined as inpatients tethered to a 500 lb pneumatic console driver. Recent introduction of a backpack sized portable driver (currently under clinical trial) has enabled patients to be discharged home and even return to work. Despite the profound presentation of these sick patients, there is a 79-87% success in bridge to transplantation. 相似文献
998.
The insecticidal potential of Galanthus nivalis agglutinin‐related lectins against hemipterans has been experimentally proven. However, the basis behind the toxicity of these lectins against hemipterans remains elusive. The present study elucidates the molecular basis behind insecticidal efficacy of Colocasia esculenta tuber agglutinin (CEA) against Bemisia tabaci and Lipaphis erysimi. Confocal microscopic analyses highlighted the binding of 25 kDa stable homodimeric lectin to insect midgut. Ligand blots followed by LC MS/MS analyses identified binding partners of CEA as vacuolar ATP synthase and sarcoplasmic endoplasmic reticulum type Ca2+ ATPase from B. tabaci, and ATP synthase, heat shock protein 70 and clathrin heavy chain assembly protein from L. erysimi. Internalization of CEA into hemolymph was confirmed by Western blotting. Glycoprotein nature of the receptors was identified through glycospecific staining. Deglycosylation assay indicated the interaction of CEA with its receptors to be probably glycan mediated. Surface plasmon resonance analysis revealed the interaction kinetics between ATP synthase of B. tabaci with CEA. Pathway prediction study based on Drosophila homologs suggested the interaction of CEA with insect receptors that probably led to disruption of cellular processes causing growth retardation and loss of fecundity of target insects. Thus, the present findings strengthen our current understanding of the entomotoxic potentiality of CEA, which will facilitate its future biotechnological applications. 相似文献
999.
1000.
Wilhelm Foissner Sebastian Hess Khaled Al-Rasheid 《European journal of protistology》2010,46(1):61-73
Using standard methods, we describe two new Semispathidium species from semiterrestrial habitats of tropical Africa and central Europe. Semispathidium fraterculum nov. spec. and S. pulchrum nov. spec. differ from each other and from two Namibian (Southwest African) congeners by body size, the shape and location of the extrusomes; the shape of the macronucleus, the number of ciliary rows, the number of cilia within the rows, and the number of dikinetids comprising the dorsal brush rows. The globular resting cyst of S. fraterculum is unique in having countless granules on the inside of the external layer, forming sharp-angled rows. The distribution of these and two further, not yet described south African species indicates that the genus Semispathidium evolved in subtropical Africa. Semispathidium fraterculum and S. pulchrum are highly similar, both morphologically and ecologically, differing in mainly one important feature: the shape and location of the extrusomes used for prey capture. This suggests that they evolved from a common ancestor whose areal was divided by a vicariant event, causing an independent evolution of the split populations. We argue that this and other species pairs indicate that the vicariance speciation model is applicable to protists. 相似文献